MyTaq™ Polymerases & Mixes

MyTaq^™ Polymerases & Mixes

The MyTaq^™ product range is a new generation of very high performance PCR products, designed to deliver outstanding results on all templates, including complex genomic DNA templates.

MyTaq^™ is based on the latest technology in PCR enzyme preparation, engineered to increase affinity for DNA, resulting in significant improvements to yield, sensitivity and speed. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl₂ and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

Sensitive – incorporates MyTaq^™ DNA polymerase that exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
Convenient – includes all the components necessary for high performance PCR amplification

MyTaq DNA Polymerases Customer Review

Introduction to MyTaq

Products

“With MyTaq I was able to dramatically boost my efficienci...

UC Davis School of Veterinary Medicine, USA

MyTaq^™ HS Polymerases & Mixes

MyTaq^™ HS is a new generation of very high performance, antibody-mediated hot-start PCR products, designed to give outstanding results with complex genomic DNA templates.

MyTaq^™ HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, so resulting in significant improvements in yield, sensitivity and speed. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl₂ and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq^™ HS Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

Sensitive – incorporates MyTaq^™ HS DNA Polymerase which exhibits increased affinity for DNA; thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Specific – MyTaq^™ HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast conditions

Products

MyTaq^™ One-Step RT-PCR Kits

The MyTaq^™ One-Step RT-PCR Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that MyTaq^™ One-Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive one-step RT-PCR, perfect for all downstream applications. MyTaq^™ One-Step Kit consists of reverse transcriptase, 2x MyTaq^™ HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix.

Sensitive – incorporates a blend of high-affinity RT and novel MyTaq^™ HS DNA Polymerase, enabling amplification of low copy number targets from ≥3 pg total RNA
Efficient – novel one-step buffer system maximizes the efficiency of both the reverse transcription and PCR steps, delivering improved yield of any target
Robust – RT tolerates the higher reaction temperatures required to overcome secondary structure, giving reliable detection of even challenging and GC-rich targets
Specific – MyTaq^™ HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
Flexible – utilizes gene-specific primers for full-length reverse transcription and subsequent PCR amplification of any RNA target
Convenient – an all-in-one-tube mastermix that improves the speed, convenience and accuracy of RT-PCR

Products

MyTaq^™ One-Step RT-PCR Kit

When we compared the performance of our routine supplier’s...

University of Adelaide, Australia

MyTaq^™ DNA Polymerases

Advanced molecular biology reagents engineered for accuracy in PCR, qPCR and NGS workflows