Reaction clean-up, as well as a single- or double-sided size selection of libraries following the fragmentation, ligation and PCR steps in the next-generation sequencing (NGS) workflow.
Electropherogram and virtual gel view showing removal of unincorporated adapters and PCR primers (arrows) after using the JetSeq Clean
For right-side size selection, the fragmented sample is mixed with JetSeq Clean to a range of sample to bead ratios to recover fragments below an upper size limit. The peak electropherogram view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of smaller fragments for sequencing.
For right-side size selection, the fragmented sample is mixed with JetSeq Clean to a range of sample to bead ratios to recover fragments below an upper size limit. The peak electropherogram view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of smaller fragments for sequencing.
For left-side size selection, the fragmented sample is mixed with JetSeq Clean to a range of sample to bead ratios to recover fragments above a lower size limit. The peak electropherogram view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of larger fragments for sequencing.
For left-side size selection, the virtual gel view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of larger fragments for sequencing.
Double-side size selection, supernatant from a right-side selection step, is used for left side size selection process to exclude the largest and smallest library fragments respectively. The results illustrate that adjustment of the size selection parameters allows JetSeq Clean to recover a highly specific range of library fragments and remove unwanted reaction components prior to sequencing.
JetSeq™ Clean is a NGS library preparation clean-up system based on paramagnetic bead technology, designed for efficient purification of nucleic acid fragments in next-generation sequencing workflow. JetSeq Clean provides maximum flexibility allowing for left-, right- or double-sided size selection.
JetSeq Clean removes, salts, primers, primer-dimers and dNTPs, while library fragments are selectively bound to the magnetic particles based on their size. Purified library fragments are eluted from the magnetic particles using water or a low salt buffer and can be used directly for all downstream NGS applications. The protocol can be performed manually, or adapted to liquid handling workstations (e.g. Agilent, Beckman, Caliper, Eppendorf, Hamilton, PerkinElmer and Tecan) using 96- or 384-well formats.
| Presentation | BIO-68031: 50 mL BIO-68032: 500 mL |
| Appearance | Colourless liquid with brown precipitate |
| Application | NGS, Sanger Sequencing, PCR, Nucleic acid clean up, Fragment analysis |
| Sample type | cDNA, DNA |
| Presentation | 1 vial |
| Storage | 4 °C to 8 °C (do not freeze) |
| Mix stability | See outer label |
| Consistency | Correct double-sided size selection |
Cat. No. Size
BIO-68031 50 mL
BIO-68032 500 mL
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