MyTaq™ Blood-PCR Kit offers very fast, highly-specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants.
MyTaq Blood-PCR Kit and the corresponding kit from Supplier K were used to amplify a 70.9% GC-rich fragment of DNA. Two-fold serial dilutions (Lanes 1-12), prepared from 20% human whole blood in either EDTA (a), lithium heparin (b), sodium heparin (c) or sodium citrate (d) were used as template. The results illustrate that MyTaq Blood-PCR kit gives increased PCR yield at all template concentrations.
MyTaq Blood-PCR Kit was used to amplify a 5 kb fragment of DNA. A two-fold serial dilution, prepared from 20% human whole blood (Lanes 1-12) in lithium heparin, was used as template. HyperLadder 1kb marker (M). The results illustrate that MyTaq Blood-PCR Kit can be used to successfully amplify longer amplicons.
Species-specific primers were used to amplify targets from horse, sheep, cat and dog blood (lanes 1-4). The results illustrate that MyTaq Blood-PCR Kit delivers highly sensitive and specific amplification from blood collected from a broad range of vertebrates. HyperLadder 1kb marker (M).
A 4-plex reaction was run using a two-fold serial prepared from 10% human whole blood in lithium heparin (Lanes 1-4), primers specific for the Phe (237 bp), Myc (450 bp), EGFR (844 bp) and ATP (1.2 kb) gene targets and MyTaq Blood-PCR Kit. HyperLadder 1kb marker (M). The results illustrate that MyTaq Blood-PCR kit has excellent multiplexing capability across a broad range of template concentrations.
MyTaq™ Blood-PCR Kit is recommended for fast, specific and direct PCR from human and animal blood samples. MyTaq™ Blood-PCR Kit incorporates MyTaq™ HS DNA Polymerase, the latest generation of very high-performance polymerases unique to Meridian. Furthermore, MyTaq™ HS has an increased affinity for template DNA, giving a high PCR product yield from the most challenging templates.
The combination of a unique, inhibitor-tolerant buffer system and MyTaq™ HS DNA Polymerase, ensures the MyTaq™ Blood-PCR Kit overcomes the PCR inhibitors typically present in blood samples, including anticoagulants (EDTA, citrate and heparin). This leads to significantly increased sensitivity and PCR success rates even with demanding applications such as long amplicons and GC-rich templates In addition to supporting robust PCR amplification, the novel buffer system replaces the need for complicated extraction and purification steps or the use of additives.
The speed and high specificity of MyTaq™ Blood-PCR Kit makes it highly suited for multiplex PCR and high-throughput genotyping assays. The advanced formulation of MyTaq™ Blood-PCR Kit allows fast cycling conditions to be used, without compromising PCR specificity and yield.
| Presentation | BIO-25054: 250 x 25 µL Reactions: (Mix 5x 625 µL) | ||
| Appearance | Clear, colorless solutions | ||
| Hot start | Antibody mediated | ||
| Application | SNP genotyping, Genetic testing, Pathogen detection, Blood screening, Paternity testing | ||
| Sample type | Whole blood from different animals collected with various anticoagulants (including EDTA, citrate and heparin) | ||
| Presentation | 5 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 2x | ||
| Functional | Single distinct bands of PCR product on an agarose gel using whole human blood | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-25054 250 Reactions
Markus Zeller, AutoGenomics, California, US
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!
By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.
