Meridian Life Science Academic Site

JetSeq NGS Kits

Advanced molecular biology reagents engineered for accuracy in PCR, qPCR and NGS workflows

JetSeq Lyophilized NGS Library Prep Kits are designed to be a straightforward sustainable workflow, just add the fragmented DNA, then the adapters. They are ready-to-use, consisting of an end repair/dA-tailing mix module to convert fragmented DNA into 5’-phosphorylated and 3’-dA-tailed DNA fragments, a ligase module, for the addition of sequencing adapters and an optional high-fidelity PCR module for library amplification.

  • Lyophilized reactions in sealed pouches for ambient temperature stability, transport and storage
  • Ready-to-use, just add DNA
  • Cost-effective, sustainable and accessible testing for NGS

Accurate quantification of the number of amplifiable library molecules loading onto the flow cell is a critical step in the NGS workflow in obtaining high-quality read data. The JetSeq Library Quantification Kit provides all of the components, including JetSeq FAST SYBR® mix, primers and DNA standards of known concentration to allow quantification of library DNA.

  • Accurate - qPCR-based assay for quantification of only adapter-ligated library molecules, thereby enabling optimal flow cell loading for maximum data yield and quality.
  • Sensitive - reliable quantification of even low-yield libraries, ideal for both PCR and PCR-free library preparation methods
  • Fast - delivers accurate assay results in as little as 90 minutes, thereby reducing time to results.
  • Convenient - contains a series of six pre-diluted DNA standards for rapid, simple standard curve development.
  • Economical - contains sufficient reagents and standards to quantify eighteen individual libraries on separate plates.