TetroScript Reverse Transcriptase is a robust thermostable MMLV-RT, designed to reduce RNase H activity and increase thermal stability. The enzyme offers higher cDNA yields and improved
Performance of Meridian’s TetroScript Reverse Transcriptase (red), standard MMLV-RT (grey) and a thermostable reverse transcriptase from supplier X (black) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. The data illustrates the increased thermal stability of TetroScript Reverse Transcriptase when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.
Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding.
Meridian TetroScript Reverse Transcriptase has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.
| Reagent | 10,000 units 4 x 10,000 units |
| TetroScript Reverse Transcriptase 5x Reaction Buffer |
50 µL 4 x 50 µL
1 mL 4 x 1 mL |
| Storage & Stability | TetroScript Reverse Transcriptase can be stored for up to 12 months at -20°C. Repeated freeze/thaw cycles should be avoided.When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label. |
| Shipping Conditions | On dry or blue ice |
Cat. No. Size
BIO-65111 10,000 Units
BIO-65112 4 x 10,000 Units
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