TetroScript Reverse Transcriptase is a robust thermostable MMLV-RT, designed to reduce RNase H activity and increase thermal stability. The enzyme offers higher cDNA yields and improved
Performance of Meridian’s TetroScript Reverse Transcriptase (red), standard MMLV-RT (grey) and a thermostable reverse transcriptase from supplier X (black) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. The data illustrates the increased thermal stability of TetroScript Reverse Transcriptase when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.
Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding.
Meridian TetroScript Reverse Transcriptase has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.
| Presentation | BIO-65111 10,000 Units (RT 50 µL, Buffer 1 mL) BIO-65112 40,000 Units (RT 4x 50 µL, Buffer 4x 1 mL) |
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| Appearance | Clear, colorless solutions | ||
| Application | Construction of cDNA libraries, 2- Step qPCR assays, viral RNA targets, gene cloning | ||
| Sample type | Total RNA or mRNA | ||
| Presentation | 2 vials / 8 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Functional | Primer extension analysis against a reference enzyme | ||
| Specific Activity | ≥ 300U/µg | ||
| Purity | Densitometric analysis of SDS-Page. Purity must be > 95% | ||
| DNA Contamination | None detected in qPCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. | ||
| DNase Contamination | No detectable degradation | ||
| RNase Contamination | No detectable activity | ||
Cat. No. Size
BIO-65111 10,000 Units
BIO-65112 4 x 10,000 Units
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