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5x MyTaq™ Reaction Buffer Red

5x MyTaq™ Reaction Buffer Red is an advanced formulation buffer for use with MyTaq™ DNA Polymerase.

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5x MyTaq™ Reaction Buffer Red

Increased sensitivity and speed - combined with MyTaq and MyTaq HS yield and speed are significantly improved

All in one - includes preoptimized concentrations of dNTPs, MgCl2 stabilizers and enhancers

Robust - gives high yield across a wide range of templates

Direct gel loading - for high throughput

Product Description

5x MyTaq™ Reaction Buffer Red is an advanced formulation buffer that saves time and delivers superior results. The buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations which eliminate the need for further optimization.

The specially designed red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.

Applications

  • Specific amplification of complex templates
  • Robust amplification of GC-rich sequences
  • High throughput PCR
  • Colony PCR
  • Multiplexing
  • Genotyping
  • Two-Step RT-PCR
  • TA cloning

Product Specification

Presentation BIO-37112: 4 x 1 mL
Appearance Red
Application Optimizing PCR reactions, Loading of samples onto DNA agarose gels, monitoring migration rate during agarose electrophoresis
Sample type DNA
Presentation 4 vials
Storage -20 °C
Specific Activity 5x
Stability See outer label
Functional Single distinct bands of PCR product on an agarose gel and sensitivity when used with a polymerase
DNA contamination No detectable activity
DNase contamination No detectable activity

Product Ordering

Cat. No.              Size

BIO-37112         4 x 1ml

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FAQs:

5x MyTaq™ Reaction Buffer Red

When using a Reaction Buffer Red or a Red Mix, is there a need to use loading buffer when loading PCR samples onto a gel?
No, the dyes and composition of the Red Reaction Buffers and Mixes are such that the samples will sink easily into the well and the samples can be clearly seen, thus no loading buffer is required to load your samples on an agarose gel.
Will the dye present in the colored buffers interfere with downstream applications?
The dye present in the Red Mixes or Red Reaction Buffers does not interfere with PCR product isolation procedures or subsequent applications like sequencing. An exception is the quantification of PCR products in colored buffers with photometric methods or fluorescence assays. We cannot exclude the impact of the dye on quantification results and suggest the purification of these samples, for instance with ISOLATE II PCR & Gel kit or SureClean Plus.

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