A new generation of hot-start polymerase that delivers improved specificity, yield, speed and robustness when amplifying targets from any template.
A 340 bp (A) and a 450 bp (B) fragment of the myc gene, a 525 bp (C) fragment of the EGFR gene and a 530 bp (D) fragment of the AGRI1 gene were amplified using MyTaq™ HS and hot-start DNA polymerases from Suppliers F, K, G and S. Each polymerase was used to set-up PCR reactions containing either 100 ng, 33 ng, 10 ng, 4 ng, 1 ng, 33 pg, 10 pg and 3 pg of human genomic DNA (Lanes 1-8 respectively), prepared by a 3-fold serial dilution. Marker is HyperLadder 1kb (M). MyTaq™ HS performed well across all four human genes.
M13 vector carrying a DNA insert was cloned into E.coli cells. 2 µL increments of agar (2a) and 2 µL increments of LB (2b) were added to a series of 50 µL PCR reactions (Lanes 1-8 respectively). The results show that MyTaq™ HS DNA Polymerase was more resistant to inhibition than that of supplier S. Individual colonies were picked from a plate of E.coli, washed directly into MyTaq™ buffer and amplified using MyTaq™ HS and primers for either a 2.6 kb or an 884 bp amplicon. Marker is HyperLadder 1kb (M). The results illustrate MyTaq™ DNA Polymerase is robust and gives highly–specific results.
MyTaq™ HS Red DNA Polymerase is a new generation of antibody-mediated hot-start enzyme, engineered for highly specific and efficient amplification from even the most challenging templates. MyTaq™ HS remains inactive at room temperature allowing for convenient reaction set-up, thereby reducing non-specific amplification that can hinder PCR assays from the start. These properties make MyTaq™ HS Red DNA Polymerase the ideal choice for PCR assays containing complex and low copy number targets.
The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations in the buffer helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats. The optimized buffer system and MyTaq™ HS with its increased affinity for DNA, enables very high yield PCR amplification over a wide range of PCR templates, resulting in reliable amplification from even very low amounts of template.
The advanced formulation of MyTaq™ HS Red DNA Polymerase and buffer system has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors. Furthermore it allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield.
MyTaq™ HS Red DNA Polymerase includes a 5x MyTaq™ Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR eliminating the need to add loading buffer.
| Presentation | BIO-21115 1,000 Units (Polymerase 200 µL, Buffer 8x 1.5 mL) BIO-21116 2,500 Units (Polymerase 2x 250 µL, Buffer 14x 1.5 mL) |
||
| Appearance | Clear, colorless polymerase, red buffer solution | ||
| Hot start | Antibody mediated | ||
| Application | Fast PCR, Multiplex PCR, Genotyping, Complex templates (e.g. GC-rich), Low copy number PCR assays, High-throughput assays with prolonged PCR set-up | ||
| Sample type | DNA, cDNA | ||
| Presentation | 3 vials / 9 vials / 16 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 5 u/µL | ||
| Functional | Single band of PCR product on an agarose gel and sensitivity | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-21115 1000 Units
BIO-21116 2500 Units
Kristina Marinak, West Virginia University, Morgantown, US
Roslin Institute, Scotland
Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!
By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.
