Formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.
A 1 kb fragment was amplified in duplicate from serial dilution of mouse total RNA (30 ng, 3 ng, 300 pg, 30pg and 3 pg respectively) using RN18S-1000 primers and the MyTaq One-Step RT-PCR Kit. The completed RT-PCR reactions were analysed on a 1% agarose gel. HyperLadder 50bp (M). The results show that the MyTaq One-Step RT-PCR Kit achieves high–yield, specific amplification from even complex templates.
A 1 kb fragment was amplified in duplicate from a serial dilution of mouse total RNA (10 ng, 2 ng, 400 pg, 80 pg, 16 pg and 3 pg; lanes 1-6 respectively) using RN18S-1000 primers and the MyTaq One-Step RT-PCR Kit. HyperLadder 50bp (M). The reverse transcriptase in the MyTaq One-Step RT-PCR Kit was able to deliver high quality cDNA even at 50°C, over a broad dynamic range.
MyTaq™ One-Step RT-PCR Kit incorporates the latest advances in buffer chemistry, including Meridian ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq™ HS, a new generation of antibody-mediated hot-start DNA polymerase. This ensures that MyTaq™ One-Step RT-PCR Kit enables fast, highly-specific and ultra-sensitive amplification of RNA targets for use in a broad range of downstream applications.
MyTaq™ One-Step Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative or semi-quantitative analysis of RNA transcription levels. The one-step format uses gene specific primers to maximize amplification of these targets, whilst eliminating non-specific amplification, ensuring highly efficient and sensitive transcription from as little as 3 pg total RNA.
MyTaq™ One-Step RT-PCR Kit is perfect for the synthesis of double-stranded cDNA products for subsequent gene expression analysis over a broad temperature range, the use of higher temperatures allows reverse transcription through RNA secondary structure, including difficult and GC-rich sequences. Enhanced amplification of these targets ensures high cDNA yields from all RNA, including total RNA, mRNA, in vitro transcribed RNA, snRNA and viral RNA.
| Presentation | BIO-65048: 25 x 50 µL Reactions: (RT 12.5 µL, Mix 625 µL, RI 25 µL, Water 1.8 mL) BIO-65049: 100 x 50 µL Reactions: (RT 50 µL, Mix 2x 1.25 mL, RI 100 µL, Water 1.8 mL) |
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| Appearance | Clear, colorless solutions | ||
| Hot start | Antibody mediated | ||
| Application | Gene-expression analysis, Transcription analysis, cDNA cloning, Multiplex RT-PCR | ||
| Sample type | Total RNA, mRNA | ||
| Presentation | 4 vials / 5 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | Mix 2x, RI 10 u/µL | ||
| Functional | Single distinct bands of PCR product on an agarose gel | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
| RNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-65048 25 Reactions
BIO-65089 100 Reactions
University of Adelaide, Australia
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