A new generation of polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template.
MyTaq™ was compared with DNA polymerases form others suppliers for the amplification of a 450 bp fragment of the human myc gene (61% GC rich). Decreasing amounts of human genomic DNA were used as template (1 mg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng; lanes 1-6 respectively) in the PCR. Marker is HyperLadder 1kb (M). MyTaq™ delivers higher yield and sensitivity compared to five polymerases from alternative suppliers.
A 450bp fragment of the human myc gene (61% GC rich) was amplified under fast conditions using MyTaq with a DNA polymerase from supplier S. The PCR was performed using both enzymes and a three-fold decreasing amount of human genomic DNA as template (200 ng – 30 pg; lanes 1-8 respectively). Marker is HyperLadder 1kb (M). MyTaq™ amplifies the target more efficiently under fast conditions, resulting in higher yield, without the need for further optimization.
MyTaq™ Red DNA Polymerase is recommended for all standard PCR applications. The MyTaq™ DNA Polymerase and MyTaq™ Red Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq™ has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq™ DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq™ means it gives excellent results under fast PCR conditions.
The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq™ Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
| Presentation | BIO-21108 500 Units (Polymerase 100 µL, Buffer 4x 1 mL) BIO-21109 2,500 Units (Polymerase 2x 100 µL, Buffer 14x 1.5 mL) BIO-21110 5,000 Units (Polymerase 4x 100 µL, Buffer 9x 5 mL) |
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| Appearance | Clear, colorless polymerase, red buffer solution | ||
| Hot start | No | ||
| Application | Standard PCR, High-yield PCR, Fast PCR, Colony PCR, TA cloning | ||
| Sample type | DNA, cDNA | ||
| Presentation | 5 vials / 16 vials / 13 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 5 u/µL | ||
| Functional | Single band of PCR product on an agarose gel and sensitivity | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-21108 500 Units
BIO-21109 2500 Units
BIO-21110 5000 Units
Nora Tenis, SVI, Molecular Genetics, Fitzroy, AU
Dublin City University, Ireland
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