Accurate quantification of the number of amplifiable library molecules loading onto the flow cell is a critical step in the NGS workflow in obtaining high-quality read data. The JetSeq Library Quantification Kit provides all of the components, including JetSeq FAST SYBR® mix, primers and DNA standards of known concentration to allow quantification of library DNA.
JetSeq™ Lyophilized NGS Library Prep Kits are designed to be a straightforward sustainable workflow, just add the fragmented DNA, then the adapters. They are ready-to-use, consisting of an end repair/dA-tailing mix module to convert fragmented DNA into 5’-phosphorylated and 3’-dA-tailed DNA fragments, a ligase module, for the addition of sequencing adapters and an optional high-fidelity PCR module for library amplification.
