ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need
Genomic DNA was isolated in duplicate from E. coli, mouse tail, mouse lung, HeLa cells and 3T3 cells, using ISOLATE II Genomic DNA Kit (lanes 1-5 and 6-10 respectively) and used to amplify a fragment of the Rn 18s gene. The results illustrate the consistency between replicates and between completely different species.
Genomic DNA was isolated from hair samples of eastern lowland gorilla, western gorilla, northern plains gray langur, white-headed langur and red-shanked douc (lanes 1-5 respectively). Genomic DNA was isolated using ISOLATE II Genomic DNA Kit and a fragment amplified to illustrate the consistency in quality, purity and yield of the DNA extracted.
By combining Proteinase K lysis with the speed and convenience of silica membrane purification, ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials.
By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded issue (FFPE), insects, dental swabs, buccal swabs, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgordferi) and viral DNA (e.g. CMV) from urine.
ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.
| Presentation | 10 Preps | 50 Preps | 250 Preps |
| ISOLATE II Genomic DNA Spin Columns | 10 | 50 | 250 |
| Collection Tubes (2 mL) | 20 | 100 | 500 |
| Lysis Buffer GL | 5 mL | 20 mL | 100mL |
| Buffer G3 | 10 mL | 15 mL | 75 mL |
| Wash Buffer GW1 | 6 mL | 30 mL | 150 mL |
| Wash Buffer GW2 (concentrate) | 6 mL | 12 mL | 50 mL |
| Elution Buffer G | 13 mL | 13 mL | 60 mL |
| Proteinase K (lyophilized) | 6 mL | 30 mg | 2 x 75 mg |
| Proteinase K Buffer PR | 1.8 mL | 1.8 mL | 8 mL |
| Appearance | Colorless | ||
| Application | qPCR, End-point PCR, Southern, dot and slot blotting, Genotyping, Restriction digestion, Next generation sequencing, Bisulfite conversion/methylation analysis | ||
| Sample type | Tissue, cells, bacteria, yeast, forensic samples, serum, plasma or other body fluids. | ||
| Preparation time | Approximately 20 minutes for 4-6 preps (following lysis) | ||
| Elution volume | 60 – 100 µL | ||
| Yield | Up to 60 µg of DNA | ||
| Storage | Store dissolved Proteinase K solution at ‑20°C for up to 6 months.All other kit components at room temperature (18-25°C) | ||
| Functionality | Nucleic acid recovery >90% | ||
Cat. No. Size
BIO-52065 10 Preps
BIO-52066 50 preps
BIO-52067 250 preps
Cavan Bennett, Walter and Eliza Hall Institue of Medical Research, Melbourne, Australia
Wilson Wong, University of Sydney, Camperdown , Australia
Andre Gauchel, University Bonn, Bonn, Germany
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