Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of
A five-fold serial dilution of total RNA from mouse brain (1 μg to 10 pg) was reverse transcribed using 50 Units of Tetro Reverse Transcriptase, oligo (dT)18 abd random hexamers. The resultant cDNA was then used as template in a PCR using primers for amplification of a 700 bp fragment from mouse b-actin. Lanes 1-5 correspond to PCR product from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).
A ten-fold serial dilution of human total RNA (1 μg to 10 pg) was reverse transcribed using Tetro Reverse Transcriptase and oligo (dT)18. The resultant cDNA was then used as a template in a PCR using primers for amplification of a 470 bp fragment from human GAPDH. Lanes 1-5 correspond to PCR product from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).
Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro™ Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 5 μg of RNA (fig. 2).
Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.
Tetro™ Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.
| Presentation | 10,000 Units (RT 50 µL, Buffer 1.2 mL) | ||
| Appearance | Clear, colorless solutions | ||
| Application | Construction of cDNA libraries, 2- Step qPCR assays, generation of probes for hybridization, gene cloning | ||
| Sample type | Total RNA or mRNA | ||
| Presentation | 2 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Functional | Detection of cDNA on an agarose gel | ||
| Endonuclease contamination | None detected with super coiled DNA plasmid incubated with the RT for 1 hour at 37°C | ||
| DNase Contamination | No detectable degradation | ||
| RNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-65050 10 000 Units
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