A convenient master mix containing a new generation of polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template.
MyTaq™ was compared with DNA polymerases form others suppliers for the amplification of a 450 bp fragment of the human myc gene (61% GC rich). Decreasing amounts of human genomic DNA were used as template (1 mg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng; lanes 1-6 respectively) in the PCR. Marker is HyperLadder 1kb (M). MyTaq delivers higher yield and sensitivity compared to five polymerases from alternative suppliers.
A 450 bp fragment of the human myc gene (61% GC rich) was amplified under fast conditions using MyTaq™ with a DNA polymerase from supplier S. The PCR was performed using both enzymes and a three-fold decreasing amount of human genomic DNA as template (200 ng – 30 pg; lanes 1-8 respectively). Marker is HyperLadder 1kb (M). MyTaq™ amplifies the target more efficiently under fast conditions, resulting in higher yield, without the need for further optimization.
MyTaq™ Mix is recommended for all standard PCR applications. MyTaq™ Mix a unique combination of MyTaq™ DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq™ has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq™ DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq™ means it gives excellent results under fast PCR conditions.
The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
The combination of MyTaq™ and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.
| Presentation | BIO-25041: 200 x 50 μL Reactions: 4 x 1.25 mL BIO-25042: 1,000 x 50 μL Reactions: 20 x 1.25 mL |
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| Appearance | Clear, colorless solutions | ||
| Hot start | No | ||
| Application | Standard PCR, High-yield PCR, Fast PCR, Colony PCR, TA cloning | ||
| Sample type | DNA, cDNA | ||
| Presentation | 4 vials / 20 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 2x | ||
| Functional | Single distinct bands of PCR product on an agarose gel and sensitivity | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-25041 200 x 50µL Reactions
BIO-25042 1000 x 50µL Reactions
Laboratory of Veterinary Parasitology, Faculty of Veterinary Science, University of Sydney, Australia
INRA, Nivelle, France
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