Recent technological advances have opened the door to smaller and more compact sequencing instruments that have a reduced footprint, user-friendly interface, simplified workflow, and lower sample
The DNA from the prepared libraries were A) run on a Bioanalyzer®, the red traces representing libraries made with the lyophilized library preparation kit and the blue traces libraries made with all liquid reagents. The results illustrate that both lyophilized and liquid formulations generated libraries are of similar quality, ready for sequencing.
The DNA from the prepared libraries were quantified using a qPCR-based NGS Library Quantification Kit, with P5 and P7 primers, ®, the red representing libraries made with the lyophilized library preparation kit and the blue, libraries made with all liquid reagents. The results illustrate that both lyophilized and liquid formulations generated libraries are of similar yield, demonstrating that the lyophilized reagents match the performance of liquid reagents, producing good-yield libraries ready for sequencing.
A) The number of reads before trimming, normalized to the number of reads produced by libraries prepared with liquid reagents and expressed as a percentage. The results illustrate that that both lyophilized and liquid formulations generated libraries create comparable numbers of reads.
JetSeq™ Lyophilized NGS Library Prep Kit is designed to be a straightforward sustainable workflow, just add the fragmented DNA, then the adapters. It is a ready-to-use complete solution for sequencing-by-synthesis (SBS) protocols, consisting of an end repair/dA-tailing mix module to convert fragmented DNA into 5ʹ-phosphorylated and 3ʹ-dA-tailed DNA fragments, a ligase module, for the addition of sequencing adapters and an optional high-fidelity PCR module for library amplification.
| Presentation | BIO-68033: 8 reactions BIO-68034: 8 reactions |
| Appearance | Colourless precipitate |
| Application | Sequencing by synthesis NGS library DNA |
| Sample type | cDNA, DNA |
| Presentation | 2 pouches / 3 pouches |
| Storage | Ambient temperature |
| Mix stability | See outer label |
| Functional | Quantification using qPCR |
Cat. No. Size
BIO-68033 8 Reactions
BIO-68034 8 Reactions
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