A highly inhibitor-resistant RT-qPCR 4x master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types. The supermix maximizes
Amplification profiles of Rotavirus A (an RNA virus) in a multiplex reaction with increasing levels of stool extract, 5% (yellow), 10% (red) and 20% (black) stool extract. The results demonstrate the multiplexing capability SensiFAST Probe One-Step Direct SuperMix in the presence of inhibitors found in stool (up to 20% final volume).
Amplification profiles of inactivated influenza virus (an RNA virus) spiked into samples containing 5% sputum. The results demonstrate the capability of SensiFAST Probe One-Step Direct SuperMix (red) to have a higher tolerance to inhibitors present in sputum compared to a standard One-Step RT-qPCR master mix (black).
SensiFAST™ Probe One-Step Direct SuperMix is a 4x, combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, reverse transcriptase, RNase Inhibitor, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification directly from the most challenging samples.
The advanced buffer chemistry and enhancers has been developed for fast RT-qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, making SensiFAST Direct Probe One-Step Direct SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
| Concentration | 4x |
| Presentation | BIO-76101: 200 x 20 µL Reactions: 1 x 1 mL BIO-76105: 1000 x 20 µL Reactions: 5 x 1 mL |
| Appearance | Clear, colorless solution |
| Hot Start | Antibody mediated |
| Application | Probe-based, RT-qPCR |
| Sample type | RNA |
| Presentation | 1 vial / 5 vials |
| Storage | -20 °C |
| Mix stability | See outer label |
| Consistency | ±0.5 Ct variance between test and reference sample |
| DNA Contamination | Quantitative PCR analysis with no template. Presence of E. coli and mouse genomic DNA checked. Test sample must amplify in line with control sample. |
| RNase Contamination | No detectable degradation |
| DNase Contamination | No detectable degradation |
Cat. No. Size
BIO-76101 200 Reactions
BIO-76105 1000 Reactions
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